News briefing：
Detection methods of veterinary drug residues: veterinary drug residues include antibiotics, sulfonamides, hormones, furazolidone and nifurenhydrazone.
Detection of veterinary drug residues
Tags : Veterinary drug detection Detection of veterinary drug residues
Detection methods of veterinary drug residues: veterinary drug residues include antibiotics, sulfonamides, hormones, furazolidone and nifurenhydrazone.
At present, veterinary drug residues detection methods are as follows:
1. Gold Label Detection (Gold Label Detection Card)
Colloidal gold is a common labeling technology. It is a new immunolabeling technology using colloidal gold as a tracer for antigen and antibody. It has its unique advantages. In recent years, it has been widely used in various biological research. Immunoblotting techniques used in clinic use almost all of its markers. At the same time, it may be used in flow cytometry, electron microscopy, immunology, molecular biology and even biochip.
2. Enzyme-linked immunosorbent assay
Enzyme-linked immunosorbent assay (ELISA), or ELISA, is a method that combines antibodies with enzyme complexes and then detects them by color rendering. CSY-E96SY Veterinary Drug Residue Rapid Detection Instrument produced by Shenfen Instrument adopts the principle of solid-phase enzyme-linked immunosorbent assay (ELISA). It can detect amoxicillin, malachite green, sulfonamides, aflatoxins, disease diagnosis, melamine detection, enrofloxacin, ciprofloxacin, erythromycin, chloramphenicol, oxytetracycline, tetracycline and so on. Detection of toxic and harmful substances such as sulfonamides (total amount), quinolone, diethylstilbestrol and antibiotic residues. And it can connect the food safety monitoring system. Veterinary drug residue rapid detection instrument is widely used in farms, slaughterhouses, meat products processing enterprises, inspection and quarantine units.
3. High Performance Liquid Chromatography
Also known as "high-pressure liquid chromatography", "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography" and so on. High performance liquid chromatography (HPLC) is an important branch of chromatography. Using liquid as mobile phase and high pressure infusion system, mobile phases such as single solvent with different polarities or mixed solvents and buffers with different proportions are pumped into the column with stationary phase. After the components in the column are separated, they are detected by the detector, so as to realize the analysis of samples. This method has become an important application of separation analysis technology in the fields of chemistry, medicine, industry, agriculture, commodity inspection and legal inspection.
4. Gas Chromatography
can be divided into gas-solid chromatography and gas-liquid chromatography. Gas-solid chromatography means that the mobile phase is gas and the stationary phase is the method of chromatographic separation of solid substances. For example, activated carbon and silica gel are used as stationary phases. Gas-liquid chromatography means that the mobile phase is gas and the stationary phase is the method of chromatographic separation of liquid. This is a new separation and analysis technology, which has been widely used in industry, agriculture, national defense, construction and scientific research. Gas chromatography can be divided into gas-solid chromatography and gas-liquid chromatography.
5. Mass Spectrometry (MS)
That is to say, the moving ions (charged atoms, molecules or molecular fragments, including molecular ions, isotope ions, fragment ions, rearrangement ions, multi-charge ions, metastable ions, negative ions and ions-molecule interactions) are separated by electric and magnetic fields and detected by their mass-charge ratios. The composition of ions can be determined by measuring the accurate mass of ions. This is because the exact mass of a nuclide is more than a decimal number, and there will never be two nuclides of the same quality, and there will never be one nuclide whose quality is exactly an integral multiple of that of the other. The information of molecular weight, chemical structure, pyrolysis law and some interrelationship between ions formed by single molecule decomposition can be obtained by analyzing these ions.
